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Analysis Help
Analysis: Averaged firing level vs step cycle


The average firing levels at each action potential are displayed with respect to cycles on the waveform selected by the "Cycle W.F. #". That is, the cycles are evenly divided into a number of bins, then for each spike on the waveform selected by the "Spike W.F. #", the level is measured at that same location on the waveform selected by the "Amplitude W.F. #", and added to the bin corresponding to the position in the current cycle where this spike occurred. All data are added into the bins to obtain an average curve which is displayed. The parameters "W.F. amplitude delay" and "W.F. amplitude window" indicate a range of points, relative to the point where the action potential occurred, used to obtain a level reading on this waveform. The range of voltage levels can be restricted to a smaller range, by setting the parameters "Min W.F. amplitude" and "Max W.F. amplitude". Only action potentials whose associated waveform level readings stay within these bounds will be included in the average.

This graph is most useful when dealing with a differentiated waveform. Spikes on the differentiated waveform, indicating high rates of change in level on the original waveform, can be used to trigger firing level measurements on the original waveform.

If the spike trains for the waveform selected by the "Spike W.F. #" have been properly set, statistics will be given for activity on this waveform, relative to the above cycles.

 

Key sequence  Parameter Initial Value
<Esc>AGAFVA  Analysis method  Averaged firing level vs step cycle
<Esc>SF  Run file  ?
<Esc>SRS  Start of run  0 msec
<Esc>SRE  End of run  2.14748e_08 msec
<Esc>SRD  # of deleted sections  0
<Esc>SCW  Cycle W.F. #  ?
<Esc>Wn<CR>SCV  Cycle crossings  ?
<Esc>SCS  Base cycles on stop time  N
<Esc>SCT  Base cycles on spike trains  N
<Esc>SLWN  Amplitude W.F. #  0
<Esc>SLWRL  Min W.F. amplitude  -80000 mV
<Esc>SLWRU  Max W.F. amplitude  79997.6 mV
<Esc>SLWD  W.F. amplitude delay  0 msec
<Esc>SLWW  W.F. amplitude window  0.1 msec
<Esc>SSWN  Spike W.F. #  ?
<Esc>Wn<CR>SS  W.F. spikes  ?
<Esc>SGN  Normalization  Y
<Esc>SCP  Percent of cycle active  0
<Esc>SGB  # bins- graph  100
<Esc>SLWP  W.F. ampl. as % of max  N
<Esc>SGS  Start bin- graph  1
<Esc>SGC  Cycles on graph  2
<Esc>SGR  Regression degree  0
<Esc>SSWA  Base spike stats. on start  N
<Esc>SDD  Graph description  ""
<Esc>SDM  Main graph title  ""
<Esc>SDGG  Graph type  Adaptive
<Esc>SDGH  Histogram type  Adaptive
<Esc>SDGS  Std. deviation type  Adaptive
<Esc>SDGD  Diamond symbol size  5
<Esc>SDGT  Graph tag symbol  ""
<Esc>SDSA  Auto scale  Y
<Esc>SDSR  Round out scale bars  Y
<Esc>SDSXS  X scale bars  Y
<Esc>SDSYS  Y scale bars  Y
<Esc>SDSXL  Min X  0
<Esc>SDSXU  Max X  0
<Esc>SDSYL  Min Y  0
<Esc>SDSYU  Max Y  0
<Esc>SDSYHL  Min Y- hist  0
<Esc>SDSYHU  Max Y- hist  0
<Esc>SDTA  Show areas under curves  N
<Esc>SDTH  Histogram display  N
<Esc>SDTI  Interpolation  Y
<Esc>SDTX  Extend interpolation  Y
<Esc>SDTS  Display std dev  N
<Esc>SDTT  Top title display  Y
<Esc>SDUL  Sample units  mV
<Esc>SDUT  Time units  msec

 


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Copyright © 2017 G. R. Detillieux, Spinal Cord Research Centre, The University of Manitoba.