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The instantaneous frequency of action potentials occurring on the waveform selected by the "Spike W.F. #" are averaged and displayed with respect to cycles on the waveform selected by the "Cycle W.F. #". That is, the cycles are divided evenly into a number of bins, and for each spike on the former waveform, the instantaneous frequency (the inverse of the interval from the previous spike) is calculated, and added into the appropriate bin for the position in the current cycle where this spike occurred. An average curve is then calculated and displayed with one point for each bin. No frequency is calculated for the first spike of the first cycle, if no previous spike was found, in the selected range to be analysed, to permit an interval calculation. If the spike trains for the former waveform have been properly set, statistics will be given for activity on this waveform, relative to the above cycles. Also, in this case, frequencies are calculated only for spikes in the same spike train, and no frequency is calculated for the first spike in each train.
If the "Take interval after spike" option is enabled, the interval to the following spike, rather than the previous one, is calculated. No frequency is calculated for the last spike of the last cycle, if no following spike is found in the selected range. If spike trains have been set, no frequency is calculated for the last spike in each train.