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Analysis Help
Maintenance: Reframe


The Reframe operation allows you to create a new set of frames, triggered by spikes on a waveform. You have the option of using "All" of the data (the entire run), or limiting it to a smaller "Range". By reframing only a range of the run, all of the data outside of the current analysis range are simply ignored. You also have the option of creating a "New run", which is a reframed (and maybe trimmed) version of the current run, selecting "Without-W.F." to reframe (and perhaps trim) a new version of the current run without copying the waveforms, or you can "Overwrite" the current run directly.

This operation uses the same parameters as the spike-triggered waveform averaging analysis method, i.e. it is concerned with the spikes, or action potentials, on a waveform. The "Spike W.F. #" parameter selects this waveform, and you must set the spike-related waveform parameters for this waveform. Each action potential triggers a sweep from each of the waveforms in the "W.F. # list". The "W.F. avg delay" and "W.F. avg window" control the onset and duration of each sweep.

All of the sweeps from one action potential form a single frame, and a frame is generated for each action potential. All existing frames (if any) are thrown out; only the new frames are kept. The trace numbers for the sweeps in these frames are the same as the waveform numbers in the list, and the calibration information for these new traces is simply copied from their corresponding waveforms.

If you are creating a new run, you will be asked to enter the run file name for this new run. If you enter the name of an existing run file, or if you are overwriting the current run, you will be asked for confirmation before the run is overwritten. The reframing is performed in a temporary file, so you will need enough free disk space to hold the generated run, even if you are overwriting an existing run. Once the new frame file is generated, unless you selected to reframe a new run without waveforms, the waveform files are generated (and possibly trimmed) as for the Trim operation. The same caveats apply to both operations.

This operation does not preserve links; even if waveform data files are linked together in the current run, separate data files are generated for each waveform in the reframed run. The exception to this is if you are reframing the entire run, and overwriting it, then the program won't bother to copy the waveforms as they should remain as they are.

Note that neither the analysis parameters, nor the waveform parameters, are changed by this operation. If you create a new run, none of the parameter files are copied. If you trim your current run (by reframing a Range), the time-related parameters will no longer be properly set, and will eventually have to be re-entered. (These include the analysis range and the spike and cycle activity bursts for all waveforms.) Similarly, any trace-related parameters will no longer be appropriate for the new frames generated.

 


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Copyright © 2017 G. R. Detillieux, Spinal Cord Research Centre, The University of Manitoba.