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Analysis Help
Analysis: W.F. spike cross-correlation histogram


Each action potential on the waveform selected by the "Spike W.F. #", in the range to be analysed, triggers a "sweep" from the waveform selected by the "Spike corr. W.F. #". The window and delay for these sweeps are set as they are for spike triggered averages. The sweep window is divided into a number of bins, which count the number of action potentials found in each part of every sweep analysed. The resulting graph shows the average number of action potentials in each part of the sweep window.

You can limit the number of spikes counted in each sweep, to a certain number allowed before the sweep was triggered (if using a negative delay), and a certain number allowed after the sweep was triggered. This is done by setting the parameters "Corr. spikes before trigger" and "Corr. spikes after trigger".

The program performs a test of statistical significance on the peak of the graph, and indicates above the graph if the peak is significant. A significant peak is one where the peak count exceeds the mean baseline count by more than 3.29 times the root of this mean. The results of a "K test" are also shown above the graph. These tests are described in "Short term synchronization of intercostal motoneurone activity" by T.A. Sears and D. Stagg, J. Physiol. (1976), 263, pp. 357-381.

 

Key sequence  Parameter Initial Value
<Esc>AGCC  Analysis method  W.F. spike cross-correlation histogram
<Esc>SF  Run file  ?
<Esc>SRS  Start of run  0 msec
<Esc>SRE  End of run  2.14748e_08 msec
<Esc>SRD  # of deleted sections  0
<Esc>SSWN  Spike W.F. #  ?
<Esc>Wn<CR>SS  W.F. spikes  ?
<Esc>SSCN  Spike corr. W.F. #  ?
<Esc>Wn<CR>SS  W.F. corr. spikes  ?
<Esc>SAWD  W.F. avg delay  0 msec
<Esc>SAWW  W.F. avg window  ?
<Esc>SSCB  Corr. spikes before trigger  2147483647
<Esc>SSCA  Corr. spikes after trigger  2147483647
<Esc>SGB  # bins- graph  100
<Esc>SDD  Graph description  ""
<Esc>SDM  Main graph title  ""
<Esc>SDGG  Graph type  Adaptive
<Esc>SDGH  Histogram type  Adaptive
<Esc>SDGD  Diamond symbol size  5
<Esc>SDGT  Graph tag symbol  ""
<Esc>SDSA  Auto scale  Y
<Esc>SDSR  Round out scale bars  Y
<Esc>SDSXS  X scale bars  Y
<Esc>SDSYS  Y scale bars  Y
<Esc>SDSXL  Min X  0
<Esc>SDSXU  Max X  0
<Esc>SDSYL  Min Y  0
<Esc>SDSYU  Max Y  0
<Esc>SDSYHL  Min Y- hist  0
<Esc>SDSYHU  Max Y- hist  0
<Esc>SDTH  Histogram display  N
<Esc>SDTT  Top title display  Y
<Esc>SDUT  Time units  msec

 


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Copyright © 2017 G. R. Detillieux, Spinal Cord Research Centre, The University of Manitoba.