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Analysis Help
Analysis: Trace averaging based on W.F. level


A number of bins are set up for each trace in the "Trace # list", evenly dividing the range of voltage levels measured from the waveform selected by the "Amplitude W.F. #". Each frame in the range to be analysed is added in to the appropriate bin, based on the waveform level associated with the frame. The parameters "W.F. amplitude delay" and "W.F. amplitude window" indicate a range of points, relative to the point where the frame was triggered, used to obtain a level reading on the waveform.

The range of voltage levels can be restricted to a smaller range, by setting the parameters "Min W.F. amplitude" and "Max W.F. amplitude". Only frames whose associated waveform level readings stay within these bounds will be included in the average. Normally, these two parameters only restrict the range, and do not enlarge it: if the parameters are beyond the range of voltage levels in the waveform, it is the measured range which is divided into bins, not the range defined by the parameters. However, if the "Fixed W.F. level bins" option is enabled, then the two parameters above will always define the range which is divided into bins, even if it exceeds the range of levels in the waveform.

Deleted frames, and frames whose tag values are not in the "Tag list" are excluded. You can also exclude frames that occur during the inactive phase of cycles on the waveform selected by the "Cycle W.F. #", by enabling the "Active cycle phase only" option. This restricts the average to frames that occur between the start and stop times of activity in any cycle. If the "Base cycles on stop time" option is also enabled, then the opposite is true; frames occurring in the inactive phases are included, and frames in the active phases are left out.

 

Key sequence  Parameter Initial Value
<Esc>ATW  Analysis method  Trace averaging based on W.F. level
<Esc>SF  Run file  ?
<Esc>SRS  Start of run  0 msec
<Esc>SRE  End of run  2.14748e_08 msec
<Esc>SRD  # of deleted sections  0
<Esc>SCID  Phase selection delay  0 msec
<Esc>SCIW  Phase selection window  0.1 msec
<Esc>SLTA  Active cycle phase only  N
<Esc>SCW  Cycle W.F. #  ?
<Esc>Wn<CR>SCV  Cycle crossings  ?
<Esc>SCS  Base cycles on stop time  N
<Esc>SCT  Base cycles on spike trains  N
<Esc>SLWN  Amplitude W.F. #  0
<Esc>SLWRL  Min W.F. amplitude  -80000 mV
<Esc>SLWRU  Max W.F. amplitude  79997.6 mV
<Esc>SLWF  Fixed W.F. level bins  N
<Esc>SLWD  W.F. amplitude delay  0 msec
<Esc>SLWW  W.F. amplitude window  0.1 msec
<Esc>ST  Tag list  All
<Esc>SATT  Trace # list  All
<Esc>SAB  # bins- avg  ?
<Esc>SAP  Preview averaged data  N
<Esc>SDD  Graph description  ""
<Esc>SDSA  Auto scale  Y
<Esc>SDSXS  X scale bars  Y
<Esc>SDSYS  Y scale bars  Y
<Esc>SDTI  Interpolation  Y
<Esc>SDTX  Extend interpolation  Y
<Esc>SDTO  Overlay bins  N
<Esc>SDTR  Display relative levels  N
<Esc>SDTS  Display std dev  N
<Esc>SDTT  Top title display  Y
<Esc>SDUL  Sample units  mV
<Esc>SDUT  Time units  msec

 


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Copyright © 2017 G. R. Detillieux, Spinal Cord Research Centre, The University of Manitoba.