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Analysis Help
Analysis: Raw trace amplitude vs step cycle


Displacements measured for each frame, on the trace selected by the "Amplitude trace #", are displayed with respect to cycles on the waveform selected by the "Cycle W.F. #". That is, for each cycle on the latter waveform, the corresponding frames are examined. For each of these frames, two points are measured on the selected trace, and the difference is shown on the graph, at the position in the cycle where the frame was triggered.

The position of these two points in the frame's window are selected by the parameters "Trace amplitude point" and "Trace amplitude ref". The first select the actual point to be sampled, and the second selects the reference measurement location. These two parameters have corresponding "window" parameters, which can be used to select two ranges of points in the frame's window: the program will search for the maximum point, or the minimum point, in each of the two ranges, based on the setting of the "Find max trace amplitude" parameter. If either of the two "window" parameters is set to 0, only one point is used for the corresponding measurement. A line above the graph will indicate which point or range of points was used for each of the two measurements. Regardless of whether the sample level and the reference level are obtained each from a single point, or the minimum or maximum in a range of points, the reference level is subtracted from the sample level to obtain the amplitude reading for the frame.

If the "Trace amplitude ref" option is set to a negative value (e.g. -1p), then the reference level is not measured, and the sample level is taken as an absolute reading.

If the "Average trace ampl. ref." option is enabled, rather than searching for the maximum or minimum point in the "Trace ampl. ref window" for each frame, the program will instead take the average of the points in this range as the reference level.

If the "Trace ampl. integration" option is enabled, rather than searching for the maximum or minimum point in the "Trace ampl. point window" for each frame, the program will instead integrate (sum up) the points in this range. (What are actually summed up are displacements relative to the reference level measured.)

The measurements for all cycles in the range to be analysed are overlaid on the graph. Deleted frames, and frames whose tag values are not in the "Tag list" are excluded.

 

Key sequence  Parameter Initial Value
<Esc>AGTVR  Analysis method  Raw trace amplitude vs step cycle
<Esc>SF  Run file  ?
<Esc>SRS  Start of run  0 msec
<Esc>SRE  End of run  2.14748e_08 msec
<Esc>SRD  # of deleted sections  0
<Esc>ST  Tag list  All
<Esc>SCW  Cycle W.F. #  ?
<Esc>Wn<CR>SCV  Cycle crossings  ?
<Esc>SCS  Base cycles on stop time  N
<Esc>SCT  Base cycles on spike trains  N
<Esc>SCID  Phase selection delay  0 msec
<Esc>SCIW  Phase selection window  0.1 msec
<Esc>SLTN  Amplitude trace #  0
<Esc>SLTS  Trace amplitude point  ?
<Esc>SLTR  Trace amplitude ref  -0.1 msec
<Esc>SLTWS  Trace ampl. point window  0.1 msec
<Esc>SLTWR  Trace ampl. ref window  0.1 msec
<Esc>SLTWA  Average trace ampl. ref.  N
<Esc>SLTWD  Trace ampl. ref. regression degree  0
<Esc>SLTWF  Find max trace amplitude  Y
<Esc>SLTWI  Trace ampl. integration  N
<Esc>SLTWM  Average trace ampl. sample  N
<Esc>SLTP  Trace ampl. as % of max  N
<Esc>SGN  Normalization  Y
<Esc>SCP  Percent of cycle active  0
<Esc>SGC  Cycles on graph  2
<Esc>SGR  Regression degree  0
<Esc>SDD  Graph description  ""
<Esc>SDM  Main graph title  ""
<Esc>SDGG  Graph type  Adaptive
<Esc>SDGD  Diamond symbol size  5
<Esc>SDGT  Graph tag symbol  ""
<Esc>SDSA  Auto scale  Y
<Esc>SDSR  Round out scale bars  Y
<Esc>SDSXS  X scale bars  Y
<Esc>SDSYS  Y scale bars  Y
<Esc>SDSXL  Min X  0
<Esc>SDSXU  Max X  0
<Esc>SDSYL  Min Y  0
<Esc>SDSYU  Max Y  0
<Esc>SDTI  Interpolation  Y
<Esc>SDTX  Extend interpolation  Y
<Esc>SDTT  Top title display  Y
<Esc>SDUL  Sample units  mV
<Esc>SDUT  Time units  msec

 


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Copyright © 2017 G. R. Detillieux, Spinal Cord Research Centre, The University of Manitoba.