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The instantaneous frequency of action potentials occurring on the waveform selected by the "Spike W.F. #" are displayed with respect to cycles on the waveform selected by the "Cycle W.F. #". That is, for each spike on the former waveform, the instantaneous frequency (the inverse of the interval from the previous spike) is calculated, and plotted on the graph at the position in the current cycle where this spike occurred. No frequency is plotted for the first spike of the first cycle, if no previous spike was found, in the selected range to be analysed, to permit an interval calculation. If the spike trains for the former waveform have been properly set, statistics will be given for activity on this waveform, relative to the above cycles. Also, in this case, frequencies are calculated and plotted only for spikes in the same spike train, and no frequency is plotted for the first spike in each train.
If the "Take interval after spike" option is enabled, the interval to the following spike, rather than the previous one, is calculated. No frequency is plotted for the last spike of the last cycle, if no following spike is found in the selected range. If spike trains have been set, no frequency is plotted for the last spike in each train.
For this graph, and for the similar raw inter-spike interval graph, when you disable normalization and "Auto scale", you can set the lower bound and upper bound of the X axis to go beyond the start and end of each cycle and the program will display the points occurring in those areas (in the previous or next cycle). This feature will not work for the averaged graphs.