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The firing levels at each action potential occurring on the waveform selected by the "Spike W.F. #" are displayed by spike occurrence within cycles on the waveform selected by the "Cycle W.F. #". That is, the X-axis is divided into a number of "bins", corresponding to individual spike occurrences in each cycle, then for each spike on the former waveform, the level is measured at that same location on the waveform selected by the "Amplitude W.F. #", and plotted on the graph at the position (or spike number) in the current cycle where this spike occurred. For each cycle, the firing levels for all spikes falling into one of these "bins" are displayed, overlaid by data from all other cycles in the range to be analysed. You can also skip spikes, or sample spike occurrences from the end of the cycle, just like for the "W.F. averaging - spike occurrence in cycle" analysis, using the "Spikes to skip" parameter.
The parameters "W.F. amplitude delay" and "W.F. amplitude window" indicate a range of points, relative to the point where the action potential occurred, used to obtain a level reading on this waveform. The range of voltage levels can be restricted to a smaller range, by setting the parameters "Min W.F. amplitude" and "Max W.F. amplitude". Only action potentials whose associated waveform level readings stay within these bounds will be included in the graph.