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The average intervals between action potentials occurring on the waveform selected by the "Spike W.F. #" are displayed with respect to the corresponding levels on the waveform selected by the "Amplitude W.F. #". That is, the range of voltage levels on the X-axis is divided evenly into a number of bins, and for each spike on the former waveform, the interval from the previous spike is calculated, and added to the bin representing the level on the latter waveform. All data are added into the bins to obtain an average curve which is displayed.
The parameters "W.F. amplitude delay" and "W.F. amplitude window" indicate a range of points, relative to the point where the action potential occurred, used to obtain a level reading on this latter waveform. The range of voltage levels can be restricted to a smaller range, by setting the parameters "Min W.F. amplitude" and "Max W.F. amplitude". Only action potentials whose associated waveform level readings stay within these bounds will be included in the average.
No interval is plotted for the first spike in the selected range to be analysed, since a previous spike is needed to permit an interval calculation. If the spike trains for the former waveform have been properly set, intervals are calculated and plotted only for spikes in the same spike train, and no interval is plotted for the first spike in each train.
If the "Take interval after spike" option is enabled, the interval to the following spike, rather than the previous one, is calculated. No interval is plotted for the last spike in the range to be analysed, nor for the last spike in each train, when spike trains are set.
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Copyright © 2019 G. R. Detillieux, Spinal Cord Research Centre, The University of Manitoba.