Page: 41 |
The average instantaneous frequency of action potentials occurring on the waveform selected by the "Spike W.F. #" are displayed by spike occurrence within cycles on the waveform selected by the "Cycle W.F. #". That is, the X-axis is divided into a number of bins, corresponding to individual spike occurrences in each cycle, then for each spike on the former waveform, the instantaneous frequency (inverse of the interval from the previous spike) is calculated, and added to the bin for the position (or spike number) in the current cycle where this spike occurred. For each cycle, the frequencies for all spikes falling into one of these bins are calculated and added, and a single average curve is displayed. You can also skip spikes, or sample spike occurrences from the end of the cycle, just like for the "W.F. averaging - spike occurrence in cycle" analysis, using the "Spikes to skip" parameter.
No frequency is calculated for the first spike of the first cycle, if no previous spike was found, in the selected range to be analysed, to permit an interval calculation. If the spike trains for the former waveform have been properly set, statistics will be given for activity on this waveform, relative to the above cycles. Also, in this case, frequencies are calculated only for spikes in the same spike train, and no frequency is calculated for the first spike in each train.
If the "Take interval after spike" option is enabled, the interval to the following spike, rather than the previous one, is calculated. No frequency is calculated for the last spike of the last cycle, if no following spike is found in the selected range. If spike trains have been set, no frequency is calculated for the last spike in each train.