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Analysis Help
Analysis: Averaged instantaneous spike frequency vs W.F. level


The average instantaneous frequencies of action potentials occurring on the waveform selected by the "Spike W.F. #" are displayed with respect to the corresponding levels on the waveform selected by the "Amplitude W.F. #". That is, the range of voltage levels on the X-axis is divided evenly into a number of bins, and for each spike on the former waveform, the instantaneous frequency (inverse of the interval from the previous spike) is calculated, and added to the bin representing the level on the latter waveform. All data are added into the bins to obtain an average curve which is displayed.

The parameters "W.F. amplitude delay" and "W.F. amplitude window" indicate a range of points, relative to the point where the action potential occurred, used to obtain a level reading on this latter waveform. The range of voltage levels can be restricted to a smaller range, by setting the parameters "Min W.F. amplitude" and "Max W.F. amplitude". Only action potentials whose associated waveform level readings stay within these bounds will be included in the average.

No frequency is plotted for the first spike in the selected range to be analysed, since a previous spike is needed to permit an interval calculation. If the spike trains for the former waveform have been properly set, frequencies are calculated and plotted only for spikes in the same spike train, and no frequency is plotted for the first spike in each train.

If the "Take interval after spike" option is enabled, the interval to the following spike, rather than the previous one, is calculated. No frequency is plotted for the last spike in the range to be analysed, nor for the last spike in each train, when spike trains are set.

 

Key sequence  Parameter Initial Value
<Esc>AGAWFA  Analysis method  Averaged instantaneous spike frequency vs W.F. level
<Esc>SF  Run file  ?
<Esc>SRS  Start of run  0 msec
<Esc>SRE  End of run  2.14748e_08 msec
<Esc>SRD  # of deleted sections  0
<Esc>SLTA  Active cycle phase only  N
<Esc>SCW  Cycle W.F. #  ?
<Esc>Wn<CR>SCV  Cycle crossings  ?
<Esc>SCS  Base cycles on stop time  N
<Esc>SCT  Base cycles on spike trains  N
<Esc>SLWN  Amplitude W.F. #  0
<Esc>SLWRL  Min W.F. amplitude  -80000 mV
<Esc>SLWRU  Max W.F. amplitude  79997.6 mV
<Esc>SLWD  W.F. amplitude delay  0 msec
<Esc>SLWW  W.F. amplitude window  0.1 msec
<Esc>SLWF  Fixed W.F. level bins  N
<Esc>SSWN  Spike W.F. #  ?
<Esc>Wn<CR>SS  W.F. spikes  ?
<Esc>SMIA  Take interval after spike  N
<Esc>SGB  # bins- graph  100
<Esc>SLWP  W.F. ampl. as % of max  N
<Esc>SGR  Regression degree  0
<Esc>SDD  Graph description  ""
<Esc>SDM  Main graph title  ""
<Esc>SDGG  Graph type  Adaptive
<Esc>SDGH  Histogram type  Adaptive
<Esc>SDGS  Std. deviation type  Adaptive
<Esc>SDGD  Diamond symbol size  5
<Esc>SDGT  Graph tag symbol  ""
<Esc>SDSA  Auto scale  Y
<Esc>SDSR  Round out scale bars  Y
<Esc>SDSXS  X scale bars  Y
<Esc>SDSYS  Y scale bars  Y
<Esc>SDSXL  Min X  0
<Esc>SDSXU  Max X  0
<Esc>SDSYL  Min Y  0
<Esc>SDSYU  Max Y  0
<Esc>SDSYHL  Min Y- hist  0
<Esc>SDSYHU  Max Y- hist  0
<Esc>SDTA  Show areas under curves  N
<Esc>SDTH  Histogram display  N
<Esc>SDTS  Display std dev  N
<Esc>SDTT  Top title display  Y
<Esc>SDUF  Freq. units  Hz
<Esc>SDUL  Sample units  mV

 


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Copyright © 2019 G. R. Detillieux, Spinal Cord Research Centre, The University of Manitoba.